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An in vivo Method for Determination of Endosomal Distribution of Both Ligand and Asialoglycoprotein Receptor in Rat Liver

Authors
Publisher
BioMed Central
Publication Date
Volume
3
Identifiers
DOI: 10.1186/1476-5926-2-s1-s38
Keywords
  • Proceedings
Disciplines
  • Biology
  • Medicine

Abstract

1476-5926-2-S1-S38.fm ral ss BioMed CentComparative Hepatology Open AcceProceedings An in vivo Method for Determination of Endosomal Distribution of Both Ligand and Asialoglycoprotein Receptor in Rat Liver Shana R Dalton*, Robert L Wiegert and Carol A Casey Address: University of Nebraska Medical Center and Department of Veterans Affairs Medical Center, Omaha, NE, USA Email: Shana R Dalton* - [email protected]; Robert L Wiegert - [email protected]; Carol A Casey - [email protected] * Corresponding author Introduction Alcoholic liver injury is a significant medical problem throughout the world. Protein trafficking pathways, including endocytosis, appear to be especially susceptible to the deleterious effects of alcohol. Using the asialoglyc- oprotein receptor (ASGPR) as a model, we have studied ethanol-induced alterations in the process of receptor- mediated endocytosis (RME). The ASGPR, a hepatocyte- specific receptor, binds proteins that have lost their termi- nal sialic acid moiety and have exposed galactose or N- acetylgalactosamine moieties [1]. During RME many extracellular ligands are bound by specific cell surface receptors, such as the ASGPR, and internalized via a clath- rin-coated pit pathway [2]. In the endocytotic pathway, the lumen of the endosome becomes acidic allowing the receptor-ligand complexes to dissociate. The separated receptors and ligands can either be targeted for degrada- tion in lysosomes or recycled back to the cell surface. Endosomal trafficking from the cell surface to the final destination in the cell is an extremely complex process that is still not completely understood. Our previous studies using the ASGPR model have identi- fied several ethanol-induced alterations during the proc- ess of RME. Altered receptor-ligand uncoupling and endosomal acidification as well as decreased ligand bind- ing, internalization, and degradation are some of the observed alterations [3-9]. For the current study, we wanted to examine the trafficking/distributio

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