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Cloning, expression, purification, crystallization and preliminary crystallographic analysis of NifH1 from Methanocaldococcus jannaschii

Authors
Journal
Acta Crystallographica Section F Structural Biology and Crystallization Communications
1744-3091
Publisher
International Union of Crystallography
Publication Date
Volume
67
Issue
5
Identifiers
DOI: 10.1107/s1744309111007408
Keywords
  • Crystallization Communications
Disciplines
  • Biology

Abstract

Solving the structure of MjNifH1 may help in better understanding its function and may supply some clues to understanding the evolution of nitrogenase. The full-length protein with an additional His6 tag at the C-terminus was expressed, purified and crystallized by the hanging-drop vapour-diffusion method at 287 K.

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