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Nielsen, Jakob Blæsbjerg

  • Biology
  • Chemistry
  • Design
  • Medicine
  • Pharmacology


Jakob Blæsbjerg Nielsen - DTU Orbit (04/06/14) Jakob Blæsbjerg Nielsen - DTU Orbit (04/06/14) Jakob Blæsbjerg Nielsen Home address Dalgas Have 50 A,2 tv 2000, Frederiksberg Denmark Department of Systems Biology Søltofts Plads, 223, 218 2800, Kgs. Lyngby Denmark [email protected] Phone: 45252657 Fax: 4588 4148 Mobile: 6166 5792 Job title: Assistant Professor Eucaryotic Molecular Cell Biology Center for Mikrobial Biotechnology 22/04/04 to 02/06/14 Technical University of Denmark Biocentrum-DTU, 223, 226 2800, Kgs. Lyngby Denmark [email protected] Phone: 45252703 Fax: +45 4588 4148 Job title: PhD student Center for Microbial Biotechnology 25/02/12 to 07/08/13 Publications: Accurate prediction of secondary metabolite gene clusters in filamentous fungi. Biosynthetic pathways of secondary metabolites from fungi are currently subject to an intense effort to elucidate the genetic basis for these compounds due to their large potential within pharmaceutics and synthetic biochemistry. The preferred method is methodical gene deletions to identify supporting enzymes for key synthases one cluster at a time. In this study, we design and apply a DNA expression array for Aspergillus nidulans in combination with legacy data to form a comprehensive gene expression compendium. We apply a guilt-by-association-based analysis to predict the extent of the biosynthetic clusters for the 58 synthases active in our set of experimental conditions. A comparison with legacy data shows the method to be accurate in 13 of 16 known clusters and nearly accurate for the remaining 3 clusters. Furthermore, we apply a data clustering approach, which identifies cross-chemistry between physically separate gene clusters (superclusters), and validate this both with legacy data and experimentally by prediction and verification of a supercluster consisting of the synthase AN1242 and the prenyltransferase AN11080, as well as identif

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