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Transgenic mice with defined combinations of drug-inducible reprogramming factors.

Authors
  • 1
Type
Published Article
Journal
Nature biotechnology
1546-1696
Publication Date
Volume
27
Issue
2
Pages
169–171
Identifiers
DOI: 10.1038/nbt.1520
PMID: 19151700
Source
Medline

Abstract

Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.

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