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Hydroxylation of chlorinated and unchlorinated chlorophylls in vitro

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  • Biologie
  • Ddc:570
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Hydroxylation of chlorinated and unchlorinated chlorophylls in vitro. Hydroxylation of Chlorinated and Unchlorinated Chlorophylls i n vitro M . Senge*, A . Struck**, D . D ö r n e m a n n * , H . Scheer**, and H . Senger* * Fachbereich Biologie/Botanik, Philipps-Universität. D-355Ü Marburg/Lahn, Bundesrepublik Deutschland ** Botanisches Institut der Universität, Menzinger Straße 67, D-8000 München 19, Bundesrepublik Deutschland Z . Naturforsch. 43c, 515-518 (1988); received March 7, 1988 Chlorophyll Allomerization, Hydroxy-chlorophyll, Thin Layer Chromatography Chlorophyll a is hydroxylated quantitatively in the 13 :-position. when chromatographed on silica gel thin-layer plates. This was shown by using H P L C as a non-destructive method für analyzing photosyntheticpigments. The hydroxy-group of Chi R C I , a 13:-hydroxy-20-chloro-chl o . described by Dörnemann and Senger (1986) and Scheer et cd. (1986). is shown to be artificially introduced during the purification procedure by T L C . Introduction Dur ing the experiments revealing the structure of an unknown Chlorophyll , designated C h i R C I, iso- lated by thin-layer chromatography ( T L C ) from bulk Chlorophylls, a hydroxy-group was detected at the Oppos i t i on [1, 2]. It was already discussed whether this hydroxylation took place i n vivo or was an ar- tefact, induced during the Isolation procedure [1,2] . Such an assumption was suggested by divergent re- sults with H P L C Separation [3], and by preceeding investigations of Chlorophyll alterations on silica gel layers [4, 5]. The reinvestigation of this question was possible by the application of a non-destructive H P L C method for Separation and identification of Chlorophylls, together with the use of synthetic pig- ments. Materials and Methods Chlorophyl l a (Chi a ) was prepared from Scenedes- mus mutant C - 6 E and Scenedesmus obliquus W T - D 3 and prepurified according to Omata and Mura ta [6]. 20-Chloro-pheophytin a and 13

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