Affordable Access

Publisher Website

Protective Effect of Heat Shock Protein 70 Against Oxidative Stresses in Human Corneal Fibroblasts

Journal of Korean Medical Science
Korean Academy of Medical Sciences (KAMJE)
Publication Date
DOI: 10.3346/jkms.2004.19.4.591
  • Original Article
  • Biology


We evaluated DNA protection effect of heat shock protein (HSP) against cytotoxic effects of exogenous nitric oxide (NO) and reactive oxygen intermediate (ROI). Cultured human corneal fibroblasts were divided into 4 groups. Control (Group I) was not exposed to a sub-lethal heat treatment. Other 3 groups were exposed to 43℃ for 1 hr, then incubated at 37℃ during different duration (1, 6, 24 hr, Group II, III, IV, respectively). Expression pattern of HSP 70 was analyzed by Western blot. Cell viability was measured by MTT assay and the relationship between HSP 70 expression and DNA damage was examined by terminal deoxyribonucleotidyl transferase mediated dUTP-digoxigenin nick and labeling (TUNEL) stain and single cell gel electrophoresis. Expression pattern of HSP 70 was dependent on recovery times. Cell viability following heat treatment was significantly increased and the TUNEL positive cell number was decreased at 6 hr. In single cell gel electrophoresis, tail moments were increased in a dose-dependent manner by SNAP and X/XO. Following heat treatment, tail moments showed decreased significantly at 6 hr. These results suggest that induction of HSP 70 by sub-lethal heat treatment is closely related with cytoprotective effects against oxidative stresses in human corneal fibroblasts.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Protective effect of heat shock protein 70 against...

on Journal of Korean medical scie... August 2004

The role of heat shock protein 70 in the protectiv...

on European Journal of Pharmacolo... Jan 15, 2013

Protective effects of heat shock protein 70 induce...

on Scandinavian Journal of Gastro... March 2006

Role of heat shock proteins HSP70 and HSP32 in the...

on Bulletin of experimental biolo... August 2007
More articles like this..