Affordable Access

In vitro mass rearing of the knapweed nematode, Subanguina dicridis and its use as a bioherbicide

McGill University
Publication Date
  • Russian Knapweed -- Biological Control.
  • Weeds -- Biological Control.
  • Plant Nematodes.
  • Subanguina Picridis


A culture system was established for mass rearing of the Russian knapweed nematode, Subanguina picridis (Kirjanova) Brzeski. This system consisted of two parts; a shoot culture system used as the host plant source for the nematode culture, and a monoxenic nematode culture system. The nematode developed and reproduced in this system. Galls were induced on the leaves, stems, and shoot tips of cultured Russian knapweed (Acroptilon repens (L.) DC.) shoots. After 3 months in culture, the nematode number per petri dish increased from the initial 50 inoculated to 7,000-10,000, a 140 to 200 fold increase. This study represents the first time that an above-ground gall forming nematode has been propagated in vitro. It also represents a unique monoxenic nematode culture system to mass rear above-ground endoparasitic plant nematodes.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times