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Nucleotide sequences at the 5′-termini ofin vitrosynthesized RNA

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
35
Issue
1
Identifiers
DOI: 10.1016/0006-291x(69)90478-1

Abstract

Abstract The nucleotide sequences at the 5′-termini of RNA formed in the DNA-dependent RNA polymerase reaction using a calf thymus DNA template were studied. Pancreatic or T 1 ribonuclease digestion of RNA bearing a 32P-label at the 5′-termini, followed by fractionation of radioactive terminal fragments on DEAE-cellulose in 7 M urea did not show the presence of unique sequences in the nucleotide chain following the first purine nucleotide at the 5′-terminus. The distribution of sequences was independent of the secondary structure of the template DNA.

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