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Quantitative assay of erythrocyte “free” and zinc-protoporphyrin: Clinical and genetic studies

Authors
Journal
International Journal of Biochemistry
0020-711X
Publisher
Elsevier
Publication Date
Volume
12
Identifiers
DOI: 10.1016/0020-711x(80)90212-8

Abstract

Abstract 1. 1. Two new procedures are described for the quantitative assay of “free” and zinc-protoporphyrin in red blood cells. 2. 2. Major clinical findings have included the following: (i) An average of about 10% of the total erythrocyte protoporphyrin is “free” in normal humans, but about one-third or more is usually “free” in blood of normal cows, rats and rabbits, (ii) In addition to human and bovine protoporphyria, “free” protoporphyrin was increased in (a) genetic carriers (clinically normal) of bovine protoporphyria, (b) acute Pb poisoning, and (c) some patients undergoing dialysis, (iii) Significant amounts of zinc-coproporphyrin were found in the blood of several dialyzed patients and in some “normal” cows.

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