Basics of Digital Microscopy.
University of Pittsburgh-Center for Biologic Imaging, Pittsburgh, Pennsylvania.
Purdue University, West Lafayette, Indiana.
- Published Article
Current protocols in cytometry
- Publication Date
Jan 18, 2018
Modern digital microscopy combines the equipment of classical light microscopy with a computerized imaging system. The technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer file. This chapter describes limitations that are particular to each of these processes, including optical resolution, efficiency of image registration, characteristics of image file formats, and data management. Further suggestions are given which serve, in turn, to help construct a set of guidelines aimed at optimization of digital microscopic imaging. © 2018 by John Wiley & Sons, Inc. Copyright © 2018 John Wiley & Sons, Inc.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/29345327