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Basics of Digital Microscopy.

Authors
  • Wallace, Callen T1
  • Jessup, Morgan1
  • Bernas, Tytus2
  • Peña, Karina A1
  • Calderon, Michael J1
  • Loughran, Patricia A1
  • 1 University of Pittsburgh-Center for Biologic Imaging, Pittsburgh, Pennsylvania.
  • 2 Purdue University, West Lafayette, Indiana. , (India)
Type
Published Article
Journal
Current protocols in cytometry
Publication Date
Jan 18, 2018
Volume
83
Identifiers
DOI: 10.1002/cpcy.31
PMID: 29345327
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Modern digital microscopy combines the equipment of classical light microscopy with a computerized imaging system. The technique comprises image formation by optics, image registration by a camera, and saving of image data in a computer file. This chapter describes limitations that are particular to each of these processes, including optical resolution, efficiency of image registration, characteristics of image file formats, and data management. Further suggestions are given which serve, in turn, to help construct a set of guidelines aimed at optimization of digital microscopic imaging. © 2018 by John Wiley & Sons, Inc. Copyright © 2018 John Wiley & Sons, Inc.

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