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Basement membrane polarizes lectin binding sites of Drosophila larval fat body cells.

Authors
Type
Published Article
Journal
Nature
Publication Date
Volume
303
Issue
5915
Pages
340–342
Identifiers
PMID: 6406896
Source
Medline
License
Unknown

Abstract

Vertebrate epithelial cells in monolayers are asymmetrical in that their apical and basal membranes differ in morphology and function. That this cell polarity depends on the presence of tight junctions can be demonstrated by labelling one surface of a cell monolayer in culture with fluorescent lectins and lipid probes, and subsequently observing whether the labels disperse to the opposite cell surfaces. Here we report on a differential distribution of binding sites for the lectin wheat germ agglutinin (WGA) on the cells of Drosophila melanogaster larval fat body, and show that the pattern is correlated with the structural association between the cell surfaces and their overlying basement membrane.

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