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Baicalein Cardioprotection via Oxidant Scavenging and Akt-Nitric Oxide Signaling: Identification of Early Reperfusion Phase as the Critical Therapeutic Window.

Authors
  • Chang, Wei-Tien1, 2
  • Li, Chang-Qing3
  • Hsu, Chin-Wan4
  • Lee, Chunpei3
  • Huang, Hsien-Hao5
  • Yuan, Chun-Su6
  • Chen, Wen-Jone1, 2
  • Vanden Hoek, Terry L3
  • Shao, Zuo-Hui3
  • Li, Jing3
  • 1 *Department of Emergency Medicine, National Taiwan University, College of Medicine and National Taiwan University Hospital, Taipei, Taiwan, R.O.C. , (Taiwan)
  • 2 †Cardiology Section, Department of Internal Medicine, National Taiwan University, College of Medicine and National Taiwan University Hospital, Taipei, Taiwan, R.O.C. , (Taiwan)
  • 3 ‡Department of Emergency Medicine, Center for Advanced Resuscitation Medicine, University of Illinois Hospital & Health Sciences System, Chicago, IL 60612, USA.
  • 4 §Department of Emergency Medicine, Wan-Fang Hospital, Taipei Medical University, Taipei, Taiwan, R.O.C. , (Taiwan)
  • 5 ¶Department of Emergency Medicine, Taipei Veterans General Hospital and Emergency Medicine, College of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C. , (Taiwan)
  • 6 ∥Tang Center for Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2019
Volume
47
Issue
5
Pages
1043–1056
Identifiers
DOI: 10.1142/S0192415X19500538
PMID: 31311299
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Baicalein is a natural flavonoid with anti-oxidant activities protecting against ischemia/reperfusion (I/R) injury. Previous studies suggest that oxidative burst early after reperfusion accelerates cell death. We therefore investigated the critical therapeutic window of baicalein by examining the timing of baicalein treatment in relation to its oxidant modulating and cytoprotective effects. Using an established chick cardiomyocyte model of I/R, we administered baicalein at various time points after reperfusion and assessed cell viability and the profiles of reactive oxygen species (ROS), nitric oxide (NO), and Akt phosphorylation. Baicalein administered at the onset of reperfusion resulted in a concentration-dependent reduction of cell death (25 μM 48.2±1.9%, 50μM 43.8±1.5%, 100μM 36.6±2.1%, vs. I/R control 57.3±1.4%, all p<0.05). Baicalein (100μM) timely and effectively scavenged ROS burst and enhanced NO production in the early reperfusion phase. Cotreatment with NO synthase (NOS) inhibitor l-NAME (200μM) partially abrogated the cytoprotective effect. Baicalein (100μM) given after reperfusion lost protective effect in a time-dependent manner with cytoprotection completely lost if >60min. Even with only 15-min delay after reperfusion, the ROS scavenging effect was abolished and the NO enhancing effect markedly reduced. The phosphorylation of Akt, an upstream regulator of eNOS, also diminished as the delay lengthened. In conclusion, baicalein treatment after reperfusion confers cardioprotection in a concentration- and time-dependent manner. The critical therapeutic window lies in the early reperfusion phase, during which ROS scavenging and Akt-eNOS mediated NO signaling are most effective.

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