Affordable Access

Bacterial expression and purification of the Arabidopsis NADPH-cytochrome P450 reductase ATR2.

Authors
  • Hull, A K
  • Celenza, J L
Type
Published Article
Journal
Protein expression and purification
Publication Date
Apr 01, 2000
Volume
18
Issue
3
Pages
310–315
Identifiers
PMID: 10733884
Source
Medline
License
Unknown

Abstract

An N-terminally modified form of the Arabidopsis NADPH-cytochrome P450 ATR2 (ATR2mod) was expressed from the tactac promoter in Escherichia coli to obtain high yields of the enzyme. The N-terminal modification eliminates the predicted chloroplast transit peptide of ATR2 allowing for more efficient expression. ATR2mod was purified from membrane extracts using a 2',5'-ADP-agarose affinity column. The specific activity of the purified ATR2mod for cytochrome c reduction was 9.4 micromol min(-1) mg(-1) and the K(m) for cytochrome c reduction was 15 +/- 2 microM. The purified NADPH-cytochrome P450 reductase was able to support function of CYP79B2.

Report this publication

Statistics

Seen <100 times