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Bacillus Calmette Guerin triggers the IL-12/IFN-gamma axis by an IRAK-4- and NEMO-dependent, non-cognate interaction between monocytes, NK, and T lymphocytes.

Authors
  • Feinberg, Jacqueline
  • Fieschi, Claire
  • Doffinger, Rainer
  • Feinberg, Max
  • Leclerc, Tony
  • Boisson-Dupuis, Stéphanie
  • Picard, Capucine
  • Bustamante, Jacinta
  • Chapgier, Ariane
  • Filipe-Santos, Orchidée
  • Ku, Cheng-Lung
  • de Beaucoudrey, Ludovic
  • Reichenbach, Janine
  • Antoni, Guillemette
  • Baldé, Ramatoulaye
  • Alcaïs, Alexandre
  • Casanova, Jean-Laurent
Type
Published Article
Journal
European journal of immunology
Publication Date
Nov 01, 2004
Volume
34
Issue
11
Pages
3276–3284
Identifiers
PMID: 15384045
Source
Medline
License
Unknown

Abstract

The IL-12/IFN-gamma axis is crucial for protective immunity to Mycobacterium in humans and mice. Our goal was to analyze the relative contribution of various human blood cell subsets and molecules to the production of, or response to IL-12 and IFN-gamma. We designed an assay for the stimulation of whole blood by live M. bovis Bacillus Calmette-Guerin (BCG) alone, or BCG plus IL-12 or IFN-gamma, measuring IFN-gamma and IL-12 levels. We studied patients with a variety of specific inherited immunodeficiencies resulting in a lack of leukocytes, or T, B, and/or NK lymphocytes, or polymorphonuclear cells, or a lack of expression of key molecules such as HLA class II, CD40L, NF-kappaB essential modulator (NEMO), and IL-1 receptor-associated kinase-4 (IRAK-4). Patients with deficiencies in IL-12p40, IL-12 receptor beta1 chain (IL-12Rbeta1), IFN-gammaR1, IFN-gammaR2, and STAT-1 were used as internal controls. We showed that monocytes were probably the main producers of IL-12, and that NK and T cells produced similar amounts of IFN-gamma. NEMO and IRAK-4 were found to be important for IL-12 production and subsequent IFN-gamma production, while a lack of CD40L or HLA class II had no major impact on the IL-12/IFN-gamma axis. The stimulation of whole blood by live BCG thus triggers the IL-12/IFN-gamma axis by an IRAK-4- and NEMO-dependent, non-cognate interaction between monocytes, NK, and T lymphocytes.

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