Abstract The results of atmospheric deposition of heavy metals and trace elements, determined in moss Pleurozium schreberi collected around the Temelin Nuclear Power Station in the Czech Republic in 2011, are presented. The monitored area around NPP Temelin comprises 29 sampling sites located along 8 profile radii stretched as of 2, 5, 10, and 20km from the NPP. Some pollution sources are located in the study area. A total of 42 elements (Na, Mg, Al, Cl, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Zn, As, Se, Br, Rb, Sr, Zr, Mo, Ag, Cd, In, Sb, I, Cs, Ba, La, Ce, Sm, Eu, Tb, Hf, Ta, W, Au, Hg, Pb, Th, and U) were determined by epithermal instrumental neutron activation analysis with the neutron flux density Φepi=3.6×1011n/(cm2s) at the pulsed fast reactor IBR-2, FLNP, JINR. To determine the presence of radionuclides and their measurable activity in samples, laboratory gamma spectrometry was found to be the best detection method. A coaxial HPGe detector with samples in the geometry of Marinelli containers in the laboratory of Faculty of Nuclear Sciences CTU in Prague was used. To assess the influence of NPP Temelin on the Biomass in 20km radius, the mass activity of 137Cs (Bq/kg) was determined. No other short-term radionuclides have ever been identified in any spectrum.