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Non-chromogenic peptide substrates for detection of enzymes by means of capacitance changes at metal electrodes

Authors
Journal
Journal of Biochemical and Biophysical Methods
0165-022X
Publisher
Elsevier
Publication Date
Volume
15
Identifiers
DOI: 10.1016/0165-022x(87)90112-6
Keywords
  • Electrode Capacitance
  • Non-Chromogenic Peptide Substrates
  • Electrode Adsorption
  • Enzyme Activity
Disciplines
  • Biology

Abstract

Abstract A new type of substrate for enzyme detection has been developed. The substrate is non-chromogenic and is used in an assay method based on electrode adsorption. The rate of change in the electric capacitance of the electrode is monitored and taken as a measure of the substrate adsorption. Substrate adsorption is in turn proportional to substrate bulk concentration and thus subject to changes by enzymes. The new substrate introduces a new concept in enzyme detection: as it is non-chromogenic it may contain appropriate amino acids on both sides of the bond subject to enzymatic cleavage.

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