Affordable Access

Publisher Website

Signaling lymphocytic activation molecule (SLAM) is differentially expressed in human Th1 and Th2 cells

Journal of Immunological Methods
Publication Date
DOI: 10.1016/s0022-1759(00)00200-3
  • Slam
  • Cd4+T Cells
  • Differentiation
  • Th1
  • Th2
  • Biology


Abstract We have used a real-time quantitative RT-PCR technique (TaqMan, PE Biosystems) to identify genes that are differentially expressed by human polarised CD4 + T cell subsets (Th1 or Th2). The goal was to test the feasibility of the detection method in profiling the expression of a set of marker genes important for Th1 and Th2 differentiation. We demonstrate that in polarised human Th1 cells signaling lymphocytic activation molecule (SLAM), a member of the immunoglobulin superfamily, is expressed at 7–25-fold higher levels than in Th2 cells. Along with SLAM, expression of the IL-12 receptor chain β2 (IL-12Rβ2) and the IFN-γ receptor chain β (IFN-γRβ) proved to be useful molecular markers indicating the state of T cell polarisation, as previously reported. Treatment with IL-12 increased SLAM mRNA expression in T cells by 3–4-fold, whereas a number of other cytokines including PDGF-BB, IFN-αA, IFN-αA/D, IFN-β, IFN-γ or IL-9 had no effect. Stimulating T cells by co-ligating CD3 and CD28 increased SLAM protein surface expression in both Th1 and Th2 cells. In conclusion, real-time RT-PCR detection was found to be an accurate, sensitive and highly reproducible method for fast profiling of mRNA expression in Th1 and Th2 cell subsets.

There are no comments yet on this publication. Be the first to share your thoughts.