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In vivo measurements with a potassium ion-selective microelectrode based on a new bis(crown ether)

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
178
Identifiers
DOI: 10.1016/s0003-2670(00)86273-x

Abstract

Abstract Potassium ion-selective microelectrodes with a tip diameter of 10 μm are described. The ionophore used is 2,2′-bis[3,4-(15-crown-5)-2-nitrophenylcarbamoxymethyl] tetradecane in a conventional mixture with sodium tetraphenylborate, an ether and PVC. The electrode provides Nernstian response over the range 10 −5−10 −1 M potassium activity. Its selectivity is shown to be similar to that of a valinomycin-based microelectrode. After the electrode has aged for 24 h, the 95% response time at the 10 −3 M potassium ion level is 2.0 ± 0.5 s. The applicability of the bis(crown ether) electrode for measurements in vivo is proved by monitoring the changes in potassium activity in different areas of the brain of anaesthetized rats after administration of veratrine.

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