Affordable Access

Publisher Website

Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut

Authors
Journal
Clinical and Translational Allergy
2045-7022
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Identifiers
DOI: 10.1186/2045-7022-3-s3-p168
Keywords
  • Poster Presentation
Disciplines
  • Biology
  • Medicine

Abstract

Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut POSTER PRESENTATION Open Access Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut J Hindley1*, A Koid2, RG Hamilton3, R van Ree4, SA Versteeg4, SC Dreskin5, MD Chapman2, S Wunschmann2 From Food Allergy and Anaphylaxis Meeting (FAAM 2013) Nice, France. 7-9 February 2013 Background Ara h 6 has a reported seroprevalence similar to Ara h 2, a major peanut allergen. Both allergens are of similar molecular size and share 50% identity in their amino acid sequences. Their similarity has made it difficult to obtain natural Ara h 6 free of Ara h 2 for immunoreactivity studies. The objective of this study was to obtain purified natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. Methods Natural Ara h 6 was purified from peanut flour extract by affinity chromatography and size exclusion chromatogra- phy. Purified Arah 6 was analyzed by silver-stained SDS- PAGE and tandem mass spectrometry (LC/MS-MS). The immunological and biological reactivity of Ara h 6 and Ara h 2 were compared by ELISA and histamine release assays. Results SDS-PAGE of the highly purified allergen revealed a single 14.5kD band and the identity of Ara h 6 was confirmed by tandem mass spectrometry (LC/MS-MS). The purified nAra h 6 contained <0.01% traces of Ara h 2 as assessed by ELISA. Natural Ara h 6 had a lower biological activity in basophil histamine release assays than natural Ara h 2. Chimeric ELISA showed that 70 and 75% of peanut aller- gic patients (n=57) had specific-IgE to natural Ara h 2 and natural Ara h 6 respectively. Conclusion Ara h 6 is a major peanut allergen, with comparable immu- noreactivity to Ara h 2. The highly purified Ara h 6, free of Ara h 2, will be useful for diagnostic IgE antibody assays, and for molecular and cellular studies to further investigate the immunological mechanisms of peanut alle

There are no comments yet on this publication. Be the first to share your thoughts.