Purpose To report a case of spontaneous resolution of macular microhole. Design Observational case report. Methods Clinical examination and optical coherence tomography (OCT) evaluation of a patient who experienced spontaneous resolution of a macular microhole. Results A 62-year-old woman with decreased vision was found to have a full-thickness macular microhole with posterior vitreous detachment and an operculum on fundus biomicroscopy and OCT. Spontaneous resolution of the microhole was documented on clinical examination and OCT 3 weeks after the examination when the patient returned with improved vision and a small central floater. Conclusions Macular microholes can be associated with posterior vitreous detachment. Spontaneous closure of macular microholes can occur.