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Enzymaticin vitroglycosylation using peptide-N-glycosidase F

Authors
Journal
Enzyme and Microbial Technology
0141-0229
Publisher
Elsevier
Publication Date
Volume
30
Issue
6
Identifiers
DOI: 10.1016/s0141-0229(02)00045-5
Keywords
  • Research Paper
Disciplines
  • Biology

Abstract

Abstract A high yield and cost effective process for the production of glycoproteins is attainable based on a low cost prokaryotic system with a high yield production of a nonglycosylated form, which is then glycosylated in vitro. Accordingly, the enzymatic deglycosylation of glycoproteins and enzymatic glycosylation of deglycosylated proteins were investigated using peptide- N-glycosidase F. Enzymatic in vitro glycosylation was found to occur with excess maltodextrin and ammonium salt in acetone at 50°C.

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