Abstract Herein, a novel electrochemical sensor based on an acetylene black paste electrode modified with molecularly imprinted chitosan–graphene composite film for sensitive and selective detection of bisphenol A (BPA) has been developed. Several important parameters controlling the performance of the sensor were investigated and optimised. The imprinted sensor offers a fast response and sensitive BPA quantification. Under the optimal conditions, a linear range from 8.0nM to 1.0μM and 1.0 to 20μM for the detection of BPA was observed with the detection limit of 6.0nM (S/N=3). Meanwhile, the fabricated sensor showed excellent specific recognition to template molecule among the structural similarities and coexistence substances. Furthermore, this imprinted electrochemical sensor was successfully employed to detect BPA in plastic bottled drinking water and canned beverages.