Affordable Access

Publisher Website

Experimental design considerations in quantification experiments by using the internal standard technique: Cholesterol determination by gas chromatography as a case study

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
1121
Issue
1
Identifiers
DOI: 10.1016/j.chroma.2006.03.119
Keywords
  • Internal Standard
  • Modelling
  • Experimental Design
  • Doehlert Matrix
  • Quantification
  • Cholesterol
Disciplines
  • Design

Abstract

Abstract An overview on experimental design in quantification experiments using the internal standard technique is described. Particular emphasis is given to the modelling of the relationship analyte/internal standard and the estimation of the optimal amount of internal standard to be used throughout the analytical concentration range by using a uniform shell design with a minimum number of seven experiments. The described approach is demonstrated in the analysis of cholesterol in various reference materials by gas chromatography (GC), using α-5 cholestan as internal standard. The outlined approaches can be applied in the determination of any kind of analyte.

There are no comments yet on this publication. Be the first to share your thoughts.