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Protein Adsorption on Supported Phospholipid Bilayers

Authors
Journal
Journal of Colloid and Interface Science
0021-9797
Publisher
Elsevier
Publication Date
Volume
246
Issue
1
Identifiers
DOI: 10.1006/jcis.2001.8060
Keywords
  • Protein Resistance
  • Protein Adsorption
  • Lipid Bilayer
  • Supported Biomembrane
  • Higg
  • Hsa
  • Cyt C
  • Bov Hb
  • Fibrinogen
  • Fetal Bovine Serum
Disciplines
  • Biology

Abstract

Abstract Quartz crystal microbalance with dissipation (QCM-D) measurements were used to investigate the adsorption of human fibrinogen, human serum albumin, bovine hemoglobin, horse heart cytochrome c, human immunoglobulin (hIgG), and 10% fetal bovine serum on supported bilayers of egg-phosphatidylcholine (eggPC) lipids. For comparison the adsorption of fibrinogen and hIgG to eggPC bilayers was also studied with surface plasmon resonance (SPR). The supported bilayers were formed in situ by vesicle adhesion and spontaneous fusion onto a SiO2 surface. The supported lipid bilayer is highly protein resistant: The irreversible adsorption measured with the QCM-D technique was below the detection level, while reversible protein adsorption was detected for all the proteins in the range 0.3–4% of the saturation coverage on a hydrophobic thiol monolayer on gold. The adsorbed amounts were slightly higher for the SPR measurements. Possible mechanisms for the protein resistance of eggPC bilayers are briefly discussed.

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