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Iron overload and gene expression in HepG2 cells: analysis by differential display

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
469
Identifiers
DOI: 10.1016/s0014-5793(00)01280-1
Keywords
  • Differential Display
  • Hepatocyte
  • Antioxidant
  • Apolipoprotein B
  • Semaphorin Cd100
  • Aldose Reductase
Disciplines
  • Biology

Abstract

Abstract The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways.

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