Affordable Access

Publisher Website

A new method for peptidase activity measurement in serum and tissues, usingl-leu-l-leu as substrate

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
78
Issue
3
Identifiers
DOI: 10.1016/0009-8981(77)90071-7
Disciplines
  • Medicine

Abstract

Abstract Peptidase-free l-amino-acid oxidase was purified from venom of Agkistrodon caliginosus and was used for the determination of serum peptidase (EC 3.4.1.-) activity with l-Leu- l-Leu as substrate. The peptidase activity in human serum rose remarkably in liver diseases, and had a significant correlation with serum transaminase activity. The peptidase activity in serum of rats treated with CCl 4 was higher than that of normal rats. After Cellogel electrophoresis the zymogram of peptidase from human liver using di- or tripeptide as substrate appeared with three active bands; however, peptidase in serum of a patient with acute hepatitis showed only one active band.

There are no comments yet on this publication. Be the first to share your thoughts.