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Analytical and micropreparative capillary electrophoresis of the peptides from calcitonin

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
198
Issue
1
Identifiers
DOI: 10.1016/0003-2697(91)90502-k
Disciplines
  • Biology

Abstract

Abstract The capillary electrophoresis (CE) of peptide fragments from the tryptic digest of salmon calcitonin and elcatonin has been carried out in H 2O- and D 2O-based buffer solutions. Analysis in heavy water was found to be superior to that in H 2O especially at a pH or pD of 7.93. From a single CE run on elcatonin digest we were also able to harvest three pure cleavage peptides in sufficient quantity to determine each amino acid residue by protein sequencing. The order of elution from CE agreed with that predicted on the basis of net charge calculated for each peptide.

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