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Schistosoma mansoni:Vaccination of mice with 10-krad-irradiated, cryopreserved schistosomules

Authors
Journal
Experimental Parasitology
0014-4894
Publisher
Elsevier
Publication Date
Volume
57
Issue
3
Identifiers
DOI: 10.1016/0014-4894(84)90099-7
Keywords
  • Schistosoma Mansoni
  • Blood Fluke
  • Trematode
  • Digenetic
  • Mouse
  • Vaccination
  • Cryopreservation
  • Immunity
  • Irradiation Of Schistosomules

Abstract

Abstract Protection against a Schistosoma mansoni cercarial challenge was evaluated in mice immunized with a vaccine composed of 10-krad-irradiated, cryopreserved schistosomules. The level of resistance induced in C57 B1 6 or NMRI (CV) mice increased with the number of schistosomules injected. Up to 83% reduction in challenge worm burden was achieved when 5000 schistosomules were injected per mouse. Intramuscular injection of the vaccine was superior to subcutaneous. Multiple immunizations, up to 3 at 4-week intervals, did not increase the resistance induced by a single immunization. A high level of protection developed in as little as 2 weeks and was maintained through at least 12 weeks postimmunization. The vaccine irradiated with 10 krad from either a 60-cobalt or 137-cesium source induced equivalent levels of resistance, and no differences were found in the immunogenicity of vaccines comprised of organisms irradiated as cercariae or as 1- to 3-hr-old schistosomules. These findings are basic to the development of a cryopreserved, live vaccine against schistosomiasis of humans or domestic animals.

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