Affordable Access

Publisher Website

Use of cytochalasin B to distinguish between early and late events in neutrophil activation

Biochimica et Biophysica Acta (BBA) - Biomembranes
Publication Date
DOI: 10.1016/0005-2736(80)90560-x
  • Activation
  • Cytochalasin B
  • β-Glucuronidase
  • Superoxide
  • Secretion
  • Ca2+Permeability
  • (Neutrophil Membrane)


Abstract Cytochalasin B greatly enhances secretion of β-glucuronidase and generation of superoxide on stimulation of rabbit peritoneal neutrophils with the soluble chemotactic factor N- formylmethionylleucylphenylalanine (f-Met-Leu-Phe). There are smaller changes due to cytochalasin B on binding of f-Met-Leu-[ 3H]-Phe, stimulation of phosphatidylinositol turnover and the stimulated increase in the permeability of the cell membrane to Ca 2+. These latter changes are probably artefactual and arise as secondary consequences of cell stimulation. Our observations support the notion that changes in Ca 2+ permeability of membranes and stimulation of phosphatidylinositol turnover reflect early stages in the sequence of events initiated by f-Met-Leu-Phe binding to its receptor and which lead to cell activation phenomena such as secretion and superoxide production.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Use of cytochalasin B to distinguish between early...

on Biochimica et Biophysica Acta Oct 02, 1980

Polymorphisms in IL-1B distinguish between psorias...

on Journal of Investigative Derma... May 2014

The roles of cyclin A2, B1, and B2 in early and la...

on Molecular Biology of the Cell Sep 15, 2010

The Roles of Cyclin A2, B1, and B2 in Early and La...

on Molecular Biology of the Cell Sep 15, 2010
More articles like this..