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Use of cytochalasin B to distinguish between early and late events in neutrophil activation

Authors
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
0005-2736
Publisher
Elsevier
Publication Date
Volume
601
Identifiers
DOI: 10.1016/0005-2736(80)90560-x
Keywords
  • Activation
  • Cytochalasin B
  • β-Glucuronidase
  • Superoxide
  • Secretion
  • Ca2+Permeability
  • (Neutrophil Membrane)

Abstract

Abstract Cytochalasin B greatly enhances secretion of β-glucuronidase and generation of superoxide on stimulation of rabbit peritoneal neutrophils with the soluble chemotactic factor N- formylmethionylleucylphenylalanine (f-Met-Leu-Phe). There are smaller changes due to cytochalasin B on binding of f-Met-Leu-[ 3H]-Phe, stimulation of phosphatidylinositol turnover and the stimulated increase in the permeability of the cell membrane to Ca 2+. These latter changes are probably artefactual and arise as secondary consequences of cell stimulation. Our observations support the notion that changes in Ca 2+ permeability of membranes and stimulation of phosphatidylinositol turnover reflect early stages in the sequence of events initiated by f-Met-Leu-Phe binding to its receptor and which lead to cell activation phenomena such as secretion and superoxide production.

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