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O2and H2O are each the source of one O in NO−2produced from NH3byNitrosomonas:15N-NMR evidence

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
164
Issue
2
Identifiers
DOI: 10.1016/0014-5793(83)80292-0
Keywords
  • Nitrosomonas
  • 15N-Nmr
  • Nitrite
  • Ammonia
  • 18O Isotope Shift
  • Nitrification

Abstract

Abstract The exchange of 18O between H 2 18O and exogeneously added 15N 16O − 2 which occurs during oxidation of ammonia by Nitrosomonas is shown to occur one oxygen at a time. Conditions in which the exchange is diminished (notably the presence of 14NO – 2 and CCCP) allowed demonstration that water and dioxygen are each the source of one oxygen in nitrite produced from 15NH 3. The nitrate produced in the presence of 18O 2 consisted of 67 and 0% 15N 18O 16O − and 15N 18O 18O −, respectively. Analysis was made using the 18O-isotope shift in 15N-NMR.

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