Affordable Access

Detection of PRRSV in 218 field samples using six molecular methods: What we are looking for?

Authors
Publication Date
Keywords
  • Objectives<Br/>The Purpose Of This Study Was To Determine The Sensitivity And The Specificity Of Six
  • <Br/>Methods<Br/>218 Field Samples (Serum
  • Tissues) Were Collected Between 2009 And 2011 From 50 Prrsv Positive And 45 Negative Pig Herds From
  • Total Viral Rna Was Extracted From Original Samples And Stored In Aliquots At -70 °C Until Analysis
  • Rt-Pcr And Direct Sequencing Of Positive Samples Was Performed As Described Previously (Toplak Et Al
  • 2012)
  • All Field Samples Were Analyzed With Five Commercial Real-Time Rt-Pcr Kits (Named As Kit A To E) Acc
  • <Br/>Results<Br/>According To Determined 258 Nucleotides Long Sequences (Orf7) 102 Prrsv Samples Bel
  • B=8
  • C=1
  • D=1
  • E=61
  • F=8
  • G=2
  • H=4
  • I=3
  • J=4
  • K=1
  • M=8) With 85
  • 7-93
  • 8 % Nucleotide Identity Between Lineages And Four Samples Belong To Type Ii
  • In Total
  • 138 Prrsv Positive Samples Were Detected With Broad Range Of Prrsv Rna In Samples
  • The Highest Sensitivity Was Observed With Kit E (96
  • 3%) And With Kit B (94
  • 5%)
  • Followed By Conventional Rt-Pcr (87
  • 8%) And Kit D (82
  • 1%)
  • While The Lowest Sensitivity Was Observed With Kit A (55
  • 3%) And Kit C (53
  • 8%)
  • Reduced Sensitivity Was Directly Related To The Genetic Lineages
  • <Br/>Discussion And Conclusion<Br/>The Study Showed That The Performance Of Commercial Rt-Pcr Assays
  • Thus
  • These Finding Emphatise That It Is Cricial That The Manifactors Ofl Diagnostic Pcr Kits (Conventiona

Abstract

Detection of PRRSV in 218 field samples using six molecular methods: What we are looking for? - DTU Orbit (21/04/14) Detection of PRRSV in 218 field samples using six molecular methods: What we are looking for? - DTU Orbit (21/04/14) Detection of PRRSV in 218 field samples using six molecular methods: What we are looking for?. / Toplak, Ivan; Štukelj, Marina; Gracieux, Patrice; Gyula, Balka; Larsen, Lars Erik; Rauh, Rolf. Proceedings of EuroPRRS2012: Understanding and combating PRRS in Europe. Budapest, Hungary, 2012. Publication: Research › Article in proceedings – Annual report year: 2012

There are no comments yet on this publication. Be the first to share your thoughts.