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High-resolution microanalysis of biological specimens by electron energy loss spectroscopy and by electron spectroscopic imaging

Authors
Journal
Journal of Ultrastructure Research
0022-5320
Publisher
Elsevier
Publication Date
Volume
72
Issue
3
Identifiers
DOI: 10.1016/s0022-5320(80)90069-6

Abstract

The application of an imaging prism—mirror—prism electron energy filter to high-spatial-resolution and high-energy-resolution microanalysis is examined for specimens of unstained chromatin, films of hematin, sections of osteoblasts, atrial muscle, myelin sheaths, and murine leukemia viruses. The results indicate a spatial resolution of 0.3 nm in electron spectroscopic imaging with elastically scattered electrons and at least 0.75 nm using an atom-specific characteristic energy loss. In energy loss spectroscopy the best resolution obtained was 0.8 eV. The minimum mass detected was 7 × 10 −21 g or 150 atoms of phosphorus in a short portion of the phospholipid-containing membrane of a murine leukemia virus.

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