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High-pressure liquid chromatography of peptides

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
135
Issue
1
Identifiers
DOI: 10.1016/s0021-9673(00)86312-8

Abstract

Abstract Peptides varying in size from di- to decapeptide have been subjected to high-pressure liquid chromatography on Phenyl-Corasil, Poragel PN, and Poragel PS under reversed-phase conditions with acetonitrile-water mixtures. It has been found that residual silanol groups in the Phenyl-Corasil and the functional groups in the Poragels significantly influence retention. Peptides were also chromatographed on a Hydrogel IV gel filtration packing with aqueous eluants, and it was found that effects other than gel filtration play a major role in determining retention.

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