Affordable Access

Publisher Website

Effect of High Glucose Concentration on Proinsulin Biosynthesis and Conversion by Human Islets

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
248
Issue
1
Identifiers
DOI: 10.1006/bbrc.1998.8924
Keywords
  • Human Islets
  • Proinsulin Conversion
  • High Glucose Concentration

Abstract

Abstract In the present study we investigate whether glucose concentration could have an effect on proinsulin biosynthesis and processing. We cultured control human islets under chronic high and low glucose concentrations. After the culture period, islets were pulse-labeled and chased for different periods of time. Proteins from islets were collected, insulin immunoprecipitated, and analyzed by alkaline–urea gel electrophoresis. We have found an accelerated rate of proinsulin conversion by those islets exposed to high glucose concentration (at 24.4mM of glucose), but not by those islets cultured at low glucose concentration (at 5.5mM of glucose). However, we do not observe any decrease or increase on newly proinsulin synthesis in any of these conditions.

There are no comments yet on this publication. Be the first to share your thoughts.