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Rub1p Processing by Yuh1p Is Required for Wild-Type Levels of Rub1p Conjugation to Cdc53p

Authors
Publisher
American Society for Microbiology
Publication Date
Source
PMC
Keywords
  • Note
Disciplines
  • Biology

Abstract

In Saccharomyces cerevisiae, Rub1p, like ubiquitin, is conjugated to proteins. Before protein conjugation, the carboxyl-terminal asparagine residue of Rub1p is removed. Rub1p conjugation is dependent on the carboxyl-terminal processing enzyme Yuh1p, whereas Rub1p lacking the asparagine residue is conjugated without Yuh1p. Thus, Yuh1p is the major processing enzyme for Rub1p.

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