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Detection of nitric oxide by electron paramagnetic resonance spectroscopy

Authors
Journal
Free Radical Biology and Medicine
0891-5849
Publisher
Elsevier
Publication Date
Volume
49
Issue
2
Identifiers
DOI: 10.1016/j.freeradbiomed.2010.03.009
Keywords
  • Nitric Oxide
  • Epr
  • Electron Paramagnetic Resonance Spectroscopy
  • Hemoglobin
  • Free Radicals
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract Electron paramagnetic resonance (EPR) spectroscopy has been used in a number of ways to study nitric oxide chemistry and biology. As an intrinsically stable and relatively unreactive diatomic free radical, the challenges of detecting this species by EPR are somewhat different from those of transient radical species. This review gives a basic introduction to EPR spectroscopy and discusses its uses to assess and quantify nitric oxide formation in biological systems.

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