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Effect of siomycin on the acceptor site of Escherichia coli ribosomes

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
45
Issue
3
Identifiers
DOI: 10.1016/0006-291x(71)90477-3

Abstract

Abstract The binding of N-acetyl- 14C-phenylalanyl-tRNA to ribosomes pretreated with polyuridylic acid and deacylated tRNA was almost completely inhibited by tetracycline and partly by siomycin. The N-acetyl- 14C-phenylalanyl-tRNA bound to ribosomes preincubated with deacylated tRNA was found to be unreactive to puromycin. By incubating the ternary complex with G factor and GTP, however, the greater part of the bound N-acetyl- 14C-phenylalalyl-tRNA became reactive to puromycin, this amount being equivalent to that amount of N-acetyl- 14C-phenylalanyl-tRNA whose binding to deacylated tRNA-treated ribosomes is inhibited by siomycin.

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