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Molecular weight estimates of insect cholinergic receptors by radiation inactivation

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
44
Issue
1
Identifiers
DOI: 10.1016/0304-3940(84)90212-x
Keywords
  • Cholinergic Receptors
  • Insect Cns
  • Radiation Inactivation

Abstract

Abstract Molecular weight (MW) estimates for sites to which the radiolabelled cholinergic receptor probes α-bungarotoxin and N-methylscopolamine bind in CNS extracts of the cockroach Periplaneta americana have been made by radiation inactivation analysis. The MW of 77,600 determined for [ 3H]Nmethylscopolamine binding sites agrees well with published values for vertebrate muscarinic acetylcholine receptor sites. In contrast, N-[propionyl- 3H]propionylated α-bungarotoxin binds to a separate membrane component of lower MW (108,000) than previously reported values for vertebrate and insect nicotinic acetylcholine receptors. This appears to represent one or more subunits of the receptor complex, containing the recognition site.

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