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Efficiency of Thymidine Incorporation in Escherichia coli B/r as a Function of Growth Rate

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PMC
Keywords
  • Genetics And Molecular Biology

Abstract

Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase.

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