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A High-Throughput Assay for siRNA-Based Circadian Screens in Human U2OS Cells

Authors
Publisher
Public Library of Science
Publication Date
Volume
3
Issue
10
Identifiers
DOI: 10.1371/journal.pone.0003457
Keywords
  • Research Article
  • Biotechnology
  • Molecular Biology
  • Genetics And Genomics/Gene Function
Disciplines
  • Biology

Abstract

The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.

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