Affordable Access

Publisher Website

Altered expression of the DNA repair protein,N-methylpurine-DNA glycosylase (MPG), in breast cancer

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
431
Issue
1
Identifiers
DOI: 10.1016/s0014-5793(98)00697-8
Keywords
  • N-Methylpurine-Dna Glycosylase
  • Dna Repair
  • Alkylpurine
  • Breast Cancer
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Abstract We examined expression of N-methylpurine-DNA glycosylase (MPG), a DNA repair enzyme that removes N-alkylpurine damage, in normal, malignant, and immortalized breast epithelial cells, and breast cancer cell lines (MDA-MB-231, MCF7, T47D). Northern analysis showed increased expression in cancer versus normal breast epithelial cells (2–24-fold). Southern blots revealed no gene amplification or polymorphisms. Immunofluorescence, immunohistochemistry, and Western blot analysis demonstrated increased MPG protein expression in the tumor cells that correlated with elevated glycosylase activity. Since MPG overexpression has been shown to be paradoxically associated with increased susceptibility to DNA damage, up-regulation of this gene may suggest a functional role in breast carcinogenesis.

There are no comments yet on this publication. Be the first to share your thoughts.