Affordable Access

Publisher Website

Pressure-induced dissociation of tight couple ribosomes

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
267
Issue
2
Identifiers
DOI: 10.1016/0014-5793(90)80934-b
Keywords
  • Elongation Cycle
  • High Pressure
  • Light Scattering
  • Ribosome
  • Subunit Dissociation
  • Tight Couple

Abstract

Abstract Ribosomes from Escherichia coli have been shown to undergo subunit dissociation at elevated hydrostatic pressure. This holds for both crude and highly purified ribosomes. No inhibitory effect could be detected by addition of either the S100 supernatant, or tRNA, polyuridylic acid, and spermine. Light scattering experiments at pressures up to 1000 bar reveal different susceptibility of tight couple and loose couple ribosomes toward pressure dissociation. Tight couples are subjected to EF-Tu-catalyzed binding of aminoacyl-tRNA, thus yielding a model system of the elongating ribosome before the peptidyl transfer step. High pressure dissociation of this compound suggests that enzymatic binding converts tight couples into loose couples. A hypothesis referring to conformational changes during the elongation cycle is presented.

There are no comments yet on this publication. Be the first to share your thoughts.