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Fructanolytic and saccharolytic enzymes ofTreponema zioleckiistrain kT

Authors
Publisher
Elsevier Ltd
Publication Date
Volume
16
Issue
4
Identifiers
DOI: 10.1016/j.anaerobe.2010.03.003
Keywords
  • Rumen
  • Treponema
  • Endolevanase
  • β-Fructofuranosidase
  • Fructan
Disciplines
  • Biology

Abstract

Abstract Enzymes in the newly described rumen bacterium, Treponema zioleckii strain kT, capable of digesting Timothy grass fructan, inulin, and sucrose were identified and characterized. Two specific endolevanases and one non-specific β-fructofuranosidase were found in a cell-free extract. The molecular weight of the endolevanases were estimated to be 60 and 36 kDa, whereas that of β-fructofuranosidase, 87 kDa. The former of the specific enzymes was associated with the outer membrane, while the latter and the non-specific β-fructofuranosidase, with the periplasm or cytosol. The K m and V max for Timothy grass fructan degradation by endolevanase were 0.27% and 15.75 μM fructose equivalents × mg protein −1 × min −1, those for sucrose and inulin digestion by β-fructofuranosidase were 1.35 × 10 −3 M and 1.73 μM hexoses × mg protein −1 × min −1 and 1.77% and 1.83 μM hexoses × mg protein −1 × min −1, respectively.

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