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High-performance liquid chromatographic determination of fusidic acid in plasma

Authors
Journal
Journal of Chromatography B Biomedical Sciences and Applications
0378-4347
Publisher
Elsevier
Publication Date
Volume
433
Identifiers
DOI: 10.1016/s0378-4347(00)80594-0
Disciplines
  • Physics

Abstract

Abstract Fusidic acid was determined in plasma by a high-performance liquid chromatographic method. Fusidic acid was extracted from plasma with acetonitrile that was salted out with ammonium sulfate. Prior to salting out cadmium sulfate was mixed with the acetonitrile-plasma mixture to help remove interfering constituents. A 150 mm × 4.6 mm column packed with 5-μm cyanopropyl stationary phase was used for chromatography. The mobile phase was acetonitrile-20 m M sodium dihydrogenphosphate (pH 3.50) (39:61, v/v). An ultraviolet-visible detector was set at 204 nm. The presence of water in the injection solvent had a significant effect on the fusidic acid peak height. A number of clinically important acquired immunodeficiency syndrome drugs did not interfere with the fusidic acid determination. The relative standard deviation varied between 0.99 and 7.8%. A limit of detection of 200 ng/ml was obtained for a 80-μl injection.

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