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Transformed Phenotype andIn vitroFlower Neoformation in Tobacco Hairy Root Regenerants

Authors
Journal
Journal of Plant Physiology
0176-1617
Publisher
Elsevier
Publication Date
Volume
130
Identifiers
DOI: 10.1016/s0176-1617(87)80226-2
Keywords
  • Agrobacterium Rhizogenes Conn.
  • Nicotiana Tabacum L.
  • Hairy Root Regenerants
  • In Vitro Flowering
  • Tissue Culture
  • Transformed Phenotype

Abstract

Summary The transformed phenotype of Nicotiana tabacum cv. Petit Havana «hairy-root» (h-r) regenerants is characterized by morphological changes which include heterostyly, reduction of all floral organs, reduced apical dominance, shorter size, and reduced numbers of internodes. Floral neoformation in vitro in h-r thin cellular layers was higher than in the control, and the floral gradient was altered. The in vitro flowers that reached anthesis showed the same alterations as the in vivoones. Phenotypic alterations in vivo and the response of thin cellular layers in vitro are correlated with the expression of pRi T-DNA auxin genes.

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