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Preparation and properties of ano-diphenol: O2oxidoreductase from cocoa husk

Authors
Journal
Phytochemistry
0031-9422
Publisher
Elsevier
Publication Date
Volume
17
Issue
11
Identifiers
DOI: 10.1016/s0031-9422(00)88728-2
Keywords
  • Theobroma Cacao
  • Sterculiaceae
  • Cocoa
  • O-Diphenol
  • O2
  • Oxidoreductase
  • Catechol.
Disciplines
  • Biology

Abstract

Abstract A particulate preparation from cocoa husk which shows o-diphenol: O 2, oxidoreductase activity contains a copper protein moiety linked to a partially formed insoluble polyphenol polymer. The particles are easily stained with osmium tetroxide for electron microscopy and show marked o-diphenol-polymerisation properties when incubated with substrate. The activity and kinetic parameters of the particles against a number of substrates and inhibitors have been determined.

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