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Regulation of Somatic Embryo Development inPicea abiesby Abscisic Acid (ABA)

Authors
Journal
Journal of Plant Physiology
0176-1617
Publisher
Elsevier
Publication Date
Volume
132
Issue
2
Identifiers
DOI: 10.1016/s0176-1617(88)80155-x
Keywords
  • Picea Abies
  • Mature Zygotic Embryo
  • Abscisic Acid
  • Somatic Embryogenesis
  • Norway Spruce
  • Plantlet Regeneration

Abstract

Summary Embryogenic callus was derived from immature and mature zygotic embryos of Picea abies cultured in the dark on half strength LP medium containing 30 mM sucrose, 10 μM NAA and 5 μM BA. The embryogenic callus was composed of small somatic embryos which consisted of small, densely cytoplasmic cells subtended by a suspensor of long highly vacuolated cells. For further development of the somatic embryos it was essential to transfer the callus to other growth regimes. The highest yield of plandets was obtained if the calli were cultured on half strength LP medium containing 90 mM sucrose and 7.6 μM ABA for one month. During this treatment the somatic embryos accumulated lipids and also became firm and developed a smooth surface. After the ABA treatment the calli were transferred to full strength LP medium containing 60 mM sucrose. On this medium plandets started to regenerate within three weeks. As the somatic embryos developed cotyledons they were isolated and cultured individually. Plants produced according to this protocol continued to grow when placed under greenhouse conditions.

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