Abstract The anti-inflammatory activity of β-adrenoceptor blocking agents viz. K.O. 592, INPEA and propranolol was investigated against carrageenin-induced oedema in albino rats. These compounds showed potent anti-inflammatory activity which was not related to their β-adrenoceptor blocking property. Since, these compounds failed to inhibit carrageenin-induced oedema in hypophysectomized as well as in bilaterally adrenalectomized rats, it seems that their anti-inflammatory activity was due to an activation of pituitary-adrenal system. β-Adrenoceptor blocking agents Carrageenin-induced oedema Pituitary-Adrenal system.