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Visualization of Lipid Droplets inC. elegansby Light and Electron Microscopy-Chapter 3

Authors
Publisher
Elsevier Science & Technology
Identifiers
DOI: 10.1016/b978-0-12-408051-5.00003-6
Keywords
  • C. Elegans
  • Lipid Droplets
  • Fluorescent Proteins
  • Confocal Microscopy
  • Electron Microscopy
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Abstract The powerful forward and reverse genetic tools, and emerging sets of biochemical assays for fat metabolites, make Caenorhabditis elegans an attractive model organism for elucidating conserved mechanisms in fat storage. The ability to observe lipid droplets in live animals at single cell resolution offers a unique advantage for studying cellular fat storage in vivo. In this chapter, we describe transgenic technologies for expressing fluorescent lipid droplet marker proteins at near-physiological levels. Methods to visualize these markers using sensitive confocal microscopy systems are detailed. Additional methods for visualizing lipid droplets by transmission electron microscopy and detection of lipid droplet associated proteins by immunoelectron microscopy are described.

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