Affordable Access

Isolation of Escherichia coli RNA Polymerase Binding Sites on T5 and T7 DNA: Further Evidence for Sigma-Dependent Recognition of A-T-Rich DNA Sequences

Authors
Publication Date
Source
PMC
Keywords
  • Biological Sciences: Biochemistry

Abstract

Previous isolation and analysis of E. coli RNA polymerase (EC 2.7.7.6) binding sites on λ DNA had demonstrated the existence of a sigma-dependent process of recognition of A-T-rich DNA sequences. We have now extended this finding to T5 and T7 DNA and häve provided evidence for the double-strandedness of the isolated binding sites. The possible equation of these sites to the genetically defined promoters is discussed.

There are no comments yet on this publication. Be the first to share your thoughts.